Part:BBa_K3882002:Design
Elimination of AHL by E. bsuahlterminator
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
T7 promoter is a high efficiency element which can have a high protein level of LUXR inside the BL21 E. coli bacteria. High level of LUXR expressed will makes the BL21 more sensitive to AHL in the environment.
Lac operator is an inhibitory DNA element which can shut down the function of AHL sensing by inhibit the transcription of LuxR gene. Thus, the AHL release by the BL21 E. coli can not be detected when culturing at the initial steps.
pelB is a commonly used signaling peptide for locating the protein into periplasm.
pvdq is the most widely used gene in the quorum system. To block quorum system activation, we use the PVDQ protein to clean up all the AHL in the environment released by P. aeruginosa.
protein linker is a commonly used in the fused protein to avoid the protein misfolded problem and loss of function.
eGFP gene is the commonly used as a green signal fused with PVDQ to increase the stability.
6x His tag is commonly used for protein purification
T7 terminator is used for stoping the protein translation.
Source
Pseudomon asaeruginosa
References
Papaioannou,E.,Wahjudi,M.,Nadal-Jimenez,P.,Koch,G.,Setroikromo,R.,Quax,W.J.,2009. Quorum-quenching acylase reduces the virulence of Pseudomon asaeruginosa in a Caenorhabditis elegans infection model. Antimicrob. Agents Chemother. 53, 4891–4897.